Samson hurt his left knee while attempting to field a ball near the boundary ropes during the 1st T20I at the Wankhede Stadium in Mumbai.
He was taken for scans and a specialist opinion this afternoon in Mumbai by the BCCI Medical Team and has been advised rest and rehabilitation.
The All-India Senior Selection Committee has named Jitesh Sharma as replacement for Sanju Samson.
India play the second T20I against Sri Lanka in Pune on Thursday.
India's updated squad for Sri Lanka T20Is: Hardik Pandya (Captain), Ishan Kishan (wk), Ruturaj Gaikwad, Shubman Gill, Suryakumar Yadav (VC), Deepak Hooda, Rahul Tripathi, Jitesh Sharma (wk), Washington Sundar, Yuzvendra Chahal, Axar Patel, Arshdeep Singh, Harshal Patel, Umran Malik, Shivam Mavi, Mukesh Kumar. (UNI)